Frequency Distribution and Antibiotics Sensitivity of E. coli from Local Muturu Raw Cow Milk
Keywords:
Antibiotic sensitivity, frequency, muturu milkAbstract
Communication in Physical Sciences, 2023, 10(1): 31-39
Authors: Imaobong T. Adenugba, Eteyen A. Uko and A. D. Ekpo
Received: 24 March 2023/Accepted:12 September 2023
Milk is widely consumed by almost all households in Nigeria and cases of manifestation of health–related problems arising from the consumption of milk are not uncommon to this study. In this study, frequency distribution and antibiotic sensitivity of Escherichia coli from raw muturu cow milk were studied and the results showed the mean standard deviation of E. coli count was highest in MC 3 with 3.63 ± 0.77 (P<0.05). This shows that there was no significant difference (P<0.05) in the total E. coli count among the muturu cows. The frequency occurrence of E. coli was highest (23.3%) in MC 1 and MC 6 followed by MC4 (16.6%) while the least occurrence was seen in MC5(6.6%). The antibiotic susceptibility profile studied shows that ciprofloxacin had the highest sensitivity pattern of (93.3%) followed by Augmentin (83.3%) while gentamycin and tarivid had (80.0%) respectively. The isolate showed resistance to streptomycin, septum and ampicillin at (43.3%), (33.3%) and (30.0%) respectively. The study reveals the high presence of E. coli in muturu cow milk with little resistance to some antibiotics which should not be overlooked while cow herders and milk collectors should pay close attention to personal and environmental hygiene during the processing of raw cow milk.
Downloads
References
Adesiyun, A. A. (2014). Bacteriological quality and associated public health risk of pre-processed bovine milk in Trinidad. International Journal of Food Microbiology, 21, pp. 253 –261
Alaku, E. N., Collinsosn, E. K., Gashe, B. A. & Mpuchane, S. (2004). Microbiological quality of milk from two processing plants in Gaborone Botswana. Food control. 15, pp.181 –186.
Alam, M. K., Akther, S. N & Sarwar, N. S. (2017). An investigation on risk factors related to the occurrence of Escherichia coli in fluid milk marketed in Chittagong, Bangladesh. Journal of Food Quality and Hazards Control. 4, pp.9– 13.
Arena, R. L. (2004). Microbiological and Physiochemical changes in Genestoso cheese, a Spanish acid curd variety, throughout ripening. Food Control,154 ,pp.217 –279.
Bandri, A. M. Ibrahim, I. T., Mohamed, S. G., Garbi, M. I., & Arbab, M. H. (2017). Prevalence of extended – spectrum beta – lactamase (ESBL) producing E. coli and Klebsiella pneumonia isolated from raw milk samples in Al Jazirah State, Sudan. pp. 102 –112.
Brooks, J. C., Martinez, B. O., Stratton, J. V., Bianchini, A. K. & Krokstrom, R. N. (2012). Survey of raw milk cheeses for microbiological quality and prevalence of food borne pathogens. Food Microbiology. 31, pp.154 –158.
Cheesbrough, M. (2006). District laboratory practice in tropical countries. Part 2. Cambridge University Press. Pp. 245 –252.
Costanzo, N. L., Ceniti, C. P., Santoro, A. L., Clausi, M. T. & Casalinouvo, F. J. (2020). Foodborne pathogen assessment in Raw milk cheeses. International Journal of Food Sciences. 29 (9), pp.123 -156.
Ezekwe, A G. & Machebe, N.S (2005). Milk yield and composition of muturu cattle under the semi intensive system of management. Nigerian Journal Of Animal Production 32(2), pp.287-292
Gruetzmacher, T. J. (1999). Identification and control of processing variables that affect the quality and safety of fluid milk. J. Food Protect., 62, pp.625-631.
Gwaza, D.S.& Momoh, D.M.(2016). Endangers indigenous cattle breeds of Nigeria a case for their conservation and management, World ScienceNews 30, pp.68-88.
Gwaza, D.S., Yahaya, A. & Chia, S.S. (2018). Assessment of herd size and structure of the Savannah muturu on free range is relation to genetic improvement in the Benue trough of Nigeria. Research and Reports on Genetics, 2, pp. 26-32.
Hahn, G. (2006). Pathogenic bacteria in raw milk situation and significance. Proceeding of Bacteriological quality of raw milk. International Diary Federation, Brussels (Belgium), pp. 67 –83.
Hanjra, S. H. & Khan, B. B. (2017). Market quality of milk in Pakistan, Diary Technology in Pakistan. PARC. Islambad, p, 58.
Hayes, M. C., Ralyea, R. D., Murphy, S. C., Carey, N. R., Scarlett, J. M. & Boor, K. J. (2001). Identification and characterization of elevated microbial counts in bulk tank raw milk. Journal of Dairy Science, 84, pp.292-298.
Hossain, M. F. Rahman, M. T. & Kabir, S. M. (2017). Microbial Assessment of milk collected from different markets of Mymensingh, Gazipur and Sherpur districts of Bangladesh and Determination of Antimicrobial Resistance patterns of the isolated bacteria. Asian Australian Journal of Food Safety and Security.1, pp. 7- 16.
Kaper, J. B., Nataro, J. P. & Mobley, H. L. (2004). Pathogenic Escherichia coli. Nat. Rev. Microbiol., 2, pp.123-140
Karnuah, A. B., Dunga G. Wennah A. Wile, W.T., Greaves E., Varkpeh, R. Osei-Amponsal & Boethcher , P. (2018). Phenotypic Characteristics of beef cattle breads and production practice in Liberia. Tropical annual health and production, 50, pp.1287 -1294.
Maikai, B.V & Madaki, P. D (2018) Enumeration of coliform in fermented milk product (nono) sold in Samara, kaduna state, Nigeria. Sokoto Journal of veterinary Science, 16(4),pp. 50-57
Mamum, M. M., Parvej, M. S., Ahamed, S. J., Hassan, J. O. & Nazir, N. K. (2016). Prevalence and Characterization of Shigatoxigenic Escherichia coli in Broiler Birds in Mymensingh. Bangladesh Journal of Veterinary Medicine. 14 ,pp. 5 –8.
Martin, M. L., Shipman, L. D., Potter, M. E., Wachsmuth, J. G.,Wells, K. G., Hedberg, R. V., Tauxe, J. P., Davis, J. H., Arnoldi, B. C. & Tilleli, J. R. (2020). Isolation of Escherichia coli O157:H7 from dairy cattle associated with two cases of hemolytic uraemic syndrome. Lancet, 85, pp.141 –148.
Masoud, W., Vogensen, F., Lillevang, S., Abu, W., Sorensen, S. & Jakobsen, M. (2012). The fate of indigenous microbiota starter cultures, Escherichia coli, Listeria sp and Staphylococcus aureus in Danish raw and cheese determined by prosequencing and quantitative real time (QRT) – PCR. International Journal for Food Microbiology, 153, pp. 192 –202.
Masud, M. A., Fakhruzzam, M. J., Rahman, M. M., Shah, M. M. & Nazir, K. H. (2012). Isolation of Escherichia coli from apparently healthy and diarrheic calves in Dinajpur area of Bangladesh and their antibiogram. Journal of Bangladesh Society for Agricultural Science and Technology, 9 pp.45 – 48.
Murinda, S. E., L.T. Nguyen, H.M. Man & R.A. Almedia, (2004) .Detection of sorbitol negative and sorbitol-positive shiga toxin-producing Escherichia coli, Listeria monocytogenes, Campylobacter jejuni and Salmonella species in dairy farm environments. Food-borne Pathogens and Disease, 1,pp.97-104.
Naair, K. H., Rahman, M. B., Nasiruddin, K. M., Akhtar, F. K., Khan, M. F. & Islam, M. S. (2005). Antibiotic sensitivity of Escherichia coli isolated from water and its relation with plasmid profile analysis. Pakistan Journal of Biological Sciences, 8 pp, 1610 –1613.
Naqvi, Z. H. (2020). Studies on the microflora composite samples of milk routinely supplied to market with particular emphasis on their identification and sources of origin. M.Sc Thesis. W. P. A. U Lyallpur.
Ogunleye, A. O., Okunlade, A. O., Jeminlehin F. O. & Apjuwae, A. T. (2013). Antibiotic resistance in Escherichia coli isolated from healthy cattle at a major cattle market in Ibadan, Oyo State, South Western, Nigeria. African Journal of Microbiology Research, 7,pp. 4572 –4575.
Oliver, S. P., Jayarao, B. M. & Almedia, R. A. (2005). Food borne pathogens in milk and the dairy environment food safety and Public Health Implications. Foodborne Pathogens and Disease, 2, pp.1115 –1129.
Ray, B. H. & Bhuriia, A. K. (2007). Fundamental Food Microbiology. CRC Press, Boca Raton. pp. 245 –287.
Russell, J. B. & Jarvis, G. N. (2001). Practical mechanisms for interrupting the oral-fecal lifecycle of Escherichia coli. Journal of Molecular Microbiology and Biotechnology. 3 (2), pp. 265 –272.
Singh, A. K., Chhabra, D. L., Sikrodia, R. M., Shukla, S. V. & Shandra, R. O. (2018). Isolation of E. coli. From Bovine mastitis and their antibiotics sensitivity pattern. International Journal of Current Microbiology and Applied Sciences 7 (10), pp. 11 – 18.
Tabaran, A. M., Mihaiu, M. J. and Tabaran, F. L., Colobatiu, L. M. & Reget, O. P. (2017). First Study on characterization of virulence and antibiotic resistance genus in Verotoxigenic and enterotoxigenic Escherichia coli isolated from raw milk and unpasteurized traditional cheeses in Romania. Folia Microbiology, 62, pp.145 – 150.
Tanzin, T. J., Naair, K. H., Zahan, M. N., Parvej, S. M., Zesmin, K. J. & Rahman, M. T. (2016). Antibiotic resistance profile of bacteria isolated from raw milk samples of cattle and buffaloes. Journal of Advanced Veterinary and Animal Research, 3, pp. 62 – 67.
Tenaillon, O., Skurnik, D., Picard B. & Denamur, E. (2010). The population genetics of commensal Escherichia coli. Nature ReviewsMicrobiology. 8 (3), pp. 207 – 217.
Tijjani, A., Utsunomiya, Y.T., Ezekwe, A.G.,Nashiru, O. & Hanotte,O (2019) Genome sequence analysis reveals selection signature in endangered Trypanotolerant West African muturu cattle(Livestock Genomics), Frontier in Genetics 10, pp. 442.
Uddin, M. A., Hasan, M. M. & Noor, R. H. (2011). Isolation and identification of Pathogenic Escherichia coli, Klebsiella spp and Staphylococcus sp. in Raw milk samples collected from different areas of Dhaka City, Bangladesh. Stamford Journal of Microbiology, 1, pp. 19 –23.
Vogt, R. L. and Dippold, L. T. (2005). Escherichia coli O157:H7 outbreak associated with consumption of ground beef, June–July 2002. Public Health Reports. 120 (2), pp.174 –178.
Wang, L. K., Wakushima, M. J. and Aota, T. L. (2013). Specific properties of Enteropathogenic Escherichia coli isolates from diarrheal patients and comparison to strains foods and faecal specimens from cattle, swine and healthy carriers in Osaka City, Japan. Applied Environmental Microbiology 79,pp. 12 –40.
Zeinhom, M. M. and Abdel – Latef, G. K. (2014). Public health risk of some milk borne pathogens. Beni-Suet University Journal of Basic and Applied Sciences. 3, pp.209 –215.
Downloads
Published
Issue
Section
License
Copyright (c) 2023 Journal and Author
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
